Graduate Faculty Directory
John Nikelly
Department of Chemistry & Biochemistry
Education
- BA (University of Illinois)
- MS, PhD (Cornell University)
Faculty Appointments
Professor of Chemistry
Research Interest
The use of parallel columns in gas chromatography, pharmaceutical analysis by GC and HPLC, and indirect detection of UV-transparent compounds.
Synopsis
My research interests are in the use of parallel columns in gas chromatography, pharmaceutical analysis by GC and HPLC, and indirect detection of UV-transparent compounds.The components in a mixture may be identified by gas chromatographic retention times, but this normally requires making runs on two different columns. Alternatively, the analysis could be done in a single run on two columns, but this requires the use of two detectors. We are developing a system of parallel columns including a retention gap that makes possible identification with a single injection and single detector resulting in two consecutive gas chromatograms. The UV detector is by far the most common type used in HPLC.
However, there are many compounds, such as ethanol and water, that are UV-transparent and thus cannot be determined by HPLC with UV detection. We have developed an indirect photometric detection technique for determining ethanol and are currently working on a similar technique for determining water. (Indirect photometric detection involves the adding of a UV-absorbing component in the mobile phase and measuring the resulting “system peaks.”)
Publications & Presentations
- “Chiral Separation of Labetalol Stereoisomers in Human Plasma by Capillary Electrophoresis,” T. V. Goel, J. G. Nikelly, R. C. Simpson, and B. K. Matuszewski, J. Chromatogr. A, 2004, 1027 (1–2), 213.
- “Metabolism of para-aminophenol by rat hepatocytes,” Z. M. Yan, J. G. Nikelly, L. Killmer, and J. B. Tarloff, Drug Metabolism and Disposition, 2000, 28(8), 880.
- “The Use of Precolumns for Solvent Focusing in Capillary Column Gas-Chromatography,” Z. M. Yan and J. G. Nikelly, J. High Resol. Chromatog., 1994, 17, 522.
- “A Solution to Split Peaks in LC,” J. G. Nikelly, J. High Resol. Chromatog., 1994, 17, 54.
Contact Information
Office:
Robert McNeil Graduate Study and Research Center
Room # 221
Box # 29
Phone: 215.596.8848
Email: j.nikell@usp.edu
